Water and protein permeability is regulated by the glomerular epithelial slit diaphragm.
نویسندگان
چکیده
منابع مشابه
Neph1 and nephrin interaction in the slit diaphragm is an important determinant of glomerular permeability.
Neph1-deficient mice develop nephrotic syndrome at birth, indicating the importance of this protein in the development of a normal glomerular filtration barrier. While the precise subcellular localization of Neph1 remains unknown, its relationship with other components of the glomerular filtration barrier is of great interest in this field. In this paper, we localize the expression of Neph1 to ...
متن کاملA Novel Domain Regulating Degradation of the Glomerular Slit Diaphragm Protein Podocin in Cell Culture Systems
Mutations in the gene NPHS2 are the most common cause of hereditary steroid-resistant nephrotic syndrome. Its gene product, the stomatin family member protein podocin represents a core component of the slit diaphragm, a unique structure that bridges the space between adjacent podocyte foot processes in the kidney glomerulus. Dislocation and misexpression of slit diaphragm components have been d...
متن کاملRapid screening of glomerular slit diaphragm integrity in larval zebrafish.
Gene array-type experiments have identified large numbers of genes thought to be important for the integrity of the glomerular slit diaphragm. Confirmation of individual proteins has been limited by the expenses and time involved in generating transgenic or knockout mice for each candidate. We present a functional screening assay based on the clearance of a 70-kDa fluorescent dextran in another...
متن کاملNephrin is specifically located at the slit diaphragm of glomerular podocytes.
We describe here the size and location of nephrin, the first protein to be identified at the glomerular podocyte slit diaphragm. In Western blots, nephrin antibodies generated against the two terminal extracellular Ig domains of recombinant human nephrin recognized a 180-kDa protein in lysates of human glomeruli and a 150-kDa protein in transfected COS-7 cell lysates. In immunofluorescence, ant...
متن کاملPorous Substructure of the Glomerular Slit Diaphragm in the Rat and Mouse
The highly ordered, isoporous substructure of the glomerular slit diaphragm was revealed in rat and mouse kidneys fixed by perfusion with tannic acid and glutaraldehyde. The slit diaphragm was similar in both animal species and appeared as a continuous junctional band, 300-450 A wide, consistently present within all slits formed by the epithelial foot processes. The diaphragm exhibited a zipper...
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ژورنال
عنوان ژورنال: Journal of the American Society of Nephrology
سال: 1994
ISSN: 1046-6673,1533-3450
DOI: 10.1681/asn.v4121957